RNA, proteins, dyes, and low-molecularweight impurities are removed by a medium-salt wash. QIAGEN plasmid purification protocols are based on a modified alkaline lysis procedure, followed by binding of plasmid DNA to QIAGEN resin under appropriate low-salt and pH conditions. These reagents, combined with the high-quality plasmid DNA obtained from QIAGEN, QIAfilter, HiSpeed®, and EndoFree® Plasmid Kits, provide optimal transfection results (see "Ordering Information", page 59). Select the optimum kit for your requirements by visiting our online selection guide at For transfection, QIAGEN also offers the advanced PolyFect®, SuperFect®, and Effectene® transfection reagents. QIAGEN offers the most comprehensive portfolio of tailored plasmid purification kits for any scale, throughput, or downstream application. The QIAGEN Plasmid Purification Kits are based on the remarkable selectivity of patented QIAGEN resin, allowing purification of ultrapure supercoiled plasmid DNA with high yields.Īnion-exchangebased QIAGEN-tips yield transfection-grade DNA, which is highly suited for use in a broad variety of demanding applications such as transfection, in vitro transcription and translation, and enzymatic modifications. In accordance with QIAGEN's ISO-certified Quality Management System, each lot of the QIAGEN Plasmid Kit is tested against predetermined specifications to ensure consistent product quality.
Qiagen plasmic midikit 12143 pdf#
These are available online in convenient and compact PDF format at where you can find, view, and print the SDS for each QIAGEN kit and kit component. For more information, please consult the appropriate safety data sheets (SDSs). When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. We recommend all users of QIAGEN products to adhere to the NIH guidelines that have been developed for recombinant DNA experiments, or to other applicable guidelines. These products are not intended for the diagnosis, prevention, or treatment of a disease.Īll due care and attention should be exercised in the handling of the products. The QIAGEN Plasmid Kits are intended for molecular biology applications.
Other buffers and RNase A stock solution can be stored for 2 years at room temperature. Under these conditions, the components are stable for 6 months without showing any reduction in performance and quality. After adding RNase A, Buffer P1 should be stored at 28☌. The QIAGEN Plasmid Kits should be stored at room temperature. Under these conditions, the components are stable for 2 years without showing any reduction in performance and quality, unless otherwise indicated on the label. QIAGEN-tips should be stored dry and at room temperature (1525☌). QIAGEN-tip 2500 QIAGEN-tip 10000 Buffer P1 Buffer P2 Buffer P3 Buffer QBT Buffer QC Buffer QF RNase A* LyseBlue Quick-Start Protocol * Provided in a 10 mg/ml or 100 mg/ml solution. QIAGEN-tip 20 QIAGEN-tip 100 QIAGEN-tip 500 Buffer P1 Buffer P2 Buffer P3 Buffer QBT Buffer QC Buffer QF RNase A* LyseBlue® Quick-Start Protocol QIAGEN Plasmid Purification Handbook 02/2021 47 Appendix A: Agarose Gel Analysis of the Purification Procedure. 30 Protocol: Very Low-Copy Plasmid/Cosmid Purification Using QIAGEN-tip 100 or QIAGEN-tip 500. 23 Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Mega and Giga Kits.
17 Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Midi and Maxi Kits. 9 Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Mini Kit. 6 Equipment and Reagents to Be Supplied by User. QIAGEN Plasmid Mini, Midi, Maxi, Mega, and Giga Kits For purification of ultrapure, transfection-grade plasmid DNA File Info : application/pdf, 66 Pages, 2.03MB Document Document HB-1193-005 HB PlasmidPurif 0221 WW February 2021
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